THE ROLE OF THE NATURAL ANTIOXIDANTS IN THE OXIHAEMOGLOBIN OXIDATION AND THE DIMINUTION OF NITRITE CONCENTRATION

Abstract

The paper includes the study of the inhibition of the process of methemoglobinization at oxidation with nitrites in the presence of sodium dihydroxyfumarate (DFH3 Na) and resveratrol (3,4’,5-trihydroxystilben). The experimental study was carried out by treatment of the erythrocyte mass by hemolysis and exposure to nitrite. The kinetic investigations were carried out in following conditions: [Resv] = (5. 10-5 – 1. 10-3) mol/l, [DFH3 Na] = 1. 10-6 – 5. 10-6 mol/l; [HbO2 ]=1. 10-3 mol/l; pH 7,1; t = 370 C. The rate of transformation of HbO2 in the presence of resveratrol and DFH3 Na was calculated from kinetic curves of consumption of the substrate and formation of MetHb obtained spectrophotometrically (λmax= 540 nm for HbO2 and λmax=630 for MetHb). It has been found out that the introduction of resveratrol and DFH3 Na in the system HbO2 – NO2 - causes the decrease of the autooxidation factor ϕ DFH3Na approximately by 1.1 – 2.5 times and ϕresveratrol by 1.1 – 1.7 times. The time of achievement of the maximum rate of oxidation of HbO2 dζ/dτ (where ζ is the rate of transformation of HbO2 in MetHb) increases while the phase of fast oxidation of HbO2 decreases with increase of content of inhibitors. The process of interaction of nitrites with reducers (such as DFH4 , DFH3 Na, resveratrol and (+)-catechine) was carried out as well. It has been established that degree of diminishing of the concentration of nitrites in the system RedH2 -NO2 - decreases as follows: DFH4 <DFH3 Na<Resv<(+)Catechol.