Abstract:
The paper includes the study of the inhibition of the process of methemoglobinization at oxidation with nitrites
in the presence of sodium dihydroxyfumarate (DFH3
Na) and resveratrol (3,4’,5-trihydroxystilben). The experimental
study was carried out by treatment of the erythrocyte mass by hemolysis and exposure to nitrite. The kinetic investigations
were carried out in following conditions: [Resv] = (5.
10-5 – 1.
10-3) mol/l, [DFH3
Na] = 1.
10-6 – 5.
10-6 mol/l; [HbO2
]=1.
10-3
mol/l; pH 7,1; t = 370
C. The rate of transformation of HbO2
in the presence of resveratrol and DFH3
Na was calculated
from kinetic curves of consumption of the substrate and formation of MetHb obtained spectrophotometrically (λmax=
540 nm for HbO2
and λmax=630 for MetHb). It has been found out that the introduction of resveratrol and DFH3
Na in
the system HbO2 – NO2
-
causes the decrease of the autooxidation factor ϕ DFH3Na approximately by 1.1 – 2.5 times and
ϕresveratrol by 1.1 – 1.7 times. The time of achievement of the maximum rate of oxidation of HbO2
dζ/dτ (where ζ is the
rate of transformation of HbO2
in MetHb) increases while the phase of fast oxidation of HbO2
decreases with increase
of content of inhibitors. The process of interaction of nitrites with reducers (such as DFH4
, DFH3
Na, resveratrol and
(+)-catechine) was carried out as well. It has been established that degree of diminishing of the concentration of nitrites
in the system RedH2
-NO2
-
decreases as follows: DFH4
<DFH3
Na<Resv<(+)Catechol.