THE ROLE OF THE NATURAL ANTIOXIDANTS IN THE OXIHAEMOGLOBIN OXIDATION AND THE DIMINUTION OF NITRITE CONCENTRATION

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dc.contributor.author Gonța, Maria
dc.date.accessioned 2018-03-20T12:13:41Z
dc.date.available 2018-03-20T12:13:41Z
dc.date.issued 2007
dc.identifier.citation GONȚA, M. The role of the natural antioxidants in the oxihaemoglobin oxidation and the diminution of nitrite concentration. In: Cemistry Journal of Moldova. 2007, nr.1, pp. 67-77. ISSN 1857-1727. en
dc.identifier.issn 1857-1727
dc.identifier.uri http://dspace.usm.md:8080/xmlui/handle/123456789/1711
dc.description.abstract The paper includes the study of the inhibition of the process of methemoglobinization at oxidation with nitrites in the presence of sodium dihydroxyfumarate (DFH 3Na) and resveratrol (3,4’,5-trihydroxystilben). The experimental study was carried out by treatment of the erythrocyte mass by hemolysis and exposure to nitrite. The kinetic investigations were carried out in following conditions: [Resv] = (5.10-5 – 110-3) mol/l, [DFH3Na] = 1.10-6 – 5.10-6 mol/l; [HbO2]=1.10-3mol/l; pH 7,1; t = 37 0C. The rate of transformation of HbO2 in the presence of resveratrol and DFH3 Na was calculated from kinetic curves of consumption of the substrate and formation of MetHb obtained spectrophotometrically (λmax= 540 nm for HbO2 and λmax=630 for MetHb). It has been found out that the introduction of resveratrol and DFH 3Na in the system HbO2 – NO2- causes the decrease of the autooxidation factor φDFH3Na approximately by 1.1 – 2.5 times and φresveratrol by 1.1 – 1.7 times. The time of achievement of the maximum rate of oxidation of HbO2 dζ/dτ (where ζ is the rate of transformation of HbO2 in MetHb) increases while the phase of fast oxidation of HbO2 decreases with increase of content of inhibitors. The process of interaction of nitrites with reducers (such as DFH4, DFH3Na, resveratrol and (+)-catechine) was carried out as well. It has been established that degree of diminishing of the ncentration of nitrites in the system RedH2-NO2- decreases as follows: DFH4<DFH3Na<Resv<(+)Catechol. en
dc.language.iso en en
dc.publisher Academia de Știinițe a Moldovei en
dc.subject nitrite en
dc.subject haemoglobin en
dc.subject inhibitor of methemoglobinization en
dc.title THE ROLE OF THE NATURAL ANTIOXIDANTS IN THE OXIHAEMOGLOBIN OXIDATION AND THE DIMINUTION OF NITRITE CONCENTRATION en
dc.type Article en


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